Two-colour immunofluorescence marker study of pleomorphic adenomas

Histochemistry. 1990;93(5):459-68. doi: 10.1007/BF00266401.


Extensive use of two-colour immunofluorescence staining for various cell markers in pleomorphic adenoma, revealed three consistent phenotypic features: (1) keratin polypeptide No. 14, which was virtually restricted to myoepithelial cells (MEC) in normal salivary glands, appeared in a large fraction of the tumour cells, suggesting that the principal neoplastic element is derived from MEC or their immediate precursors; (2) a complex co-expression pattern of various cell markers was found, with extensive concurrence of keratin and vimentin in strands of MEC-like and myxoid tumour cells, probably reflecting different degrees of tumour cell differentiation; and (3) two phenotypically distinctive dendritic cell populations were identified, one consisting of keratin positive tumour cells and the other of HLA-DR positive but keratin negative stromal cells. The significance of these findings with regard to the histogenesis and complex morphology of pleomorphic adenoma is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoma, Pleomorphic / analysis*
  • Adult
  • Aged
  • Biomarkers, Tumor / analysis*
  • Female
  • Fluorescent Antibody Technique
  • HLA-DR Antigens / analysis
  • Humans
  • Intermediate Filament Proteins / analysis
  • Male
  • Middle Aged
  • Parotid Neoplasms / analysis*
  • S100 Proteins / analysis
  • von Willebrand Factor / analysis


  • Biomarkers, Tumor
  • HLA-DR Antigens
  • Intermediate Filament Proteins
  • S100 Proteins
  • von Willebrand Factor