Phenotypic analysis of Neurospora crassa gene deletion strains

Methods Mol Biol. 2011;722:191-8. doi: 10.1007/978-1-61779-040-9_14.

Abstract

Phenotypic analysis of Neurospora crassa knockout (KO) mutants was used as a vehicle to introduce students to laboratory research. The availability of gene deletion strains was the impetus for the development of a program designed to introduce beginning science students to basic microbiology, genetics, microscopy and beginning bioinformatics. The goal was to provide a research experience, acquire laboratory skills and phenotype hundreds of KO mutants. The data provided by the students was used to build a phenotype database at the Broad Institute at Harvard/MIT for the fungal scientific community. Each mutant analysis consists of five assays that examine growth and morphology, asexual and sexual development using wild-type (parental) strains as a reference. This information indicates how loss of each gene impacts these basic and important processes.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Deletion*
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Hyphae / physiology
  • Neurospora crassa / classification*
  • Neurospora crassa / genetics
  • Neurospora crassa / metabolism
  • Neurospora crassa / physiology*
  • Phenotype
  • Spores, Fungal / physiology

Substances

  • Fungal Proteins