In vitro reconstitution of eukaryotic ion channels using droplet interface bilayers

J Am Chem Soc. 2011 Jun 22;133(24):9370-5. doi: 10.1021/ja200128n. Epub 2011 May 27.


The ability to routinely study eukaryotic ion channels in a synthetic lipid environment would have a major impact on our understanding of how different lipids influence ion channel function. Here, we describe a straightforward, detergent-free method for the in vitro reconstitution of eukaryotic ion channels and ionotropic receptors into droplet interface bilayers and measure their electrical activity at both the macroscopic and single-channel level. We explore the general applicability of this method by reconstitution of channels from a wide range of sources including recombinant cell lines and native tissues, as well as preparations that are difficult to study by conventional methods including erythrocytes and mitochondria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Electric Conductivity
  • Erythrocytes / metabolism
  • Ion Channels / metabolism*
  • Lipid Bilayers / metabolism*
  • Mice
  • Mitochondria / metabolism


  • Ion Channels
  • Lipid Bilayers