Paper based point-of-care testing disc for multiplex whole cell bacteria analysis

Biosens Bioelectron. 2011 Jul 15;26(11):4342-8. doi: 10.1016/j.bios.2011.04.035. Epub 2011 Apr 28.


Point-of-care testing (POCT) of infectious bacterial agents offers substantial benefits for disease diagnosis, mainly by shortening the time required to obtain results and by making the test available bedside or at remote care centers. Immunochromatographic lateral flow biosensors offer a low cost, highly sensitive platform for POCT. In this article, we describe the fabrication and testing of a multiplex immuno-disc sensor for the specific detection of Pseudomonas aeruginosa and Staphylococcus aureus. Antibody conjugated gold nanoparticles were used as the signaling agents. The detection range of the bacteria lies within 500-5000 CFU/ml. The advantage of the immuno-disc sensor is that it does not require any preprocessing of biological sample and is capable of whole cell bacterial detection. We also describe the design and fabrication of a compact portable device which converts the color intensity of the gold nanoparticles that accumulate at the test region into a quantitative voltage reading proportional to the bacterial concentration in the sample. The combination of the immuno-disc and the portable color reader provides a rapid, sensitive, low cost, and quantitative tool for the detection of a panel of infectious agents present in the patient sample.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies, Bacterial
  • Antibodies, Immobilized
  • Bacterial Infections / diagnosis
  • Bacteriological Techniques / instrumentation
  • Bacteriological Techniques / methods*
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Equipment Design
  • Gold
  • Humans
  • Metal Nanoparticles
  • Paper
  • Point-of-Care Systems*
  • Pseudomonas aeruginosa / immunology
  • Pseudomonas aeruginosa / isolation & purification
  • Staphylococcus aureus / immunology
  • Staphylococcus aureus / isolation & purification


  • Antibodies, Bacterial
  • Antibodies, Immobilized
  • Gold