Current results with slow freezing and vitrification of the human oocyte

Reprod Biomed Online. 2011 Sep;23(3):314-22. doi: 10.1016/j.rbmo.2010.11.019. Epub 2010 Dec 13.


The past decade has witnessed renewed interest in human oocyte cryopreservation (OCP). This article reviews the two general methods used for OCP, slow freezing and vitrification, compares the outcomes associated with each technique and discusses the factors that might influence success with OCP (such as oocyte selection or day of transfer). Based on available data, OCP offers a reliable, reproducible method for preservation of the female gamete and will find increasing application in assisted reproductive technology. Oocyte cryopreservation can provide a number of advantages to couples undergoing assisted reproduction or to women interested in fertility preservation. Two methods, slow freezing and vitrification, have been used successfully for oocyte cryopreservation. This article reviews and compares these methods, and discusses various factors that can impact upon success of oocyte cryopreservation.

Publication types

  • Comparative Study
  • Review

MeSH terms

  • Cell Survival
  • Cryopreservation / methods*
  • Cryoprotective Agents / pharmacology
  • Embryo Implantation
  • Female
  • Fertilization in Vitro
  • Humans
  • Oocytes*
  • Pregnancy
  • Pregnancy Rate


  • Cryoprotective Agents