Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay

Clin Vaccine Immunol. 2011 Jul;18(7):1187-90. doi: 10.1128/CVI.00039-11. Epub 2011 May 18.


A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies, Viral / analysis
  • Antibodies, Viral / blood*
  • Caliciviridae Infections / diagnosis*
  • Enzyme-Linked Immunosorbent Assay
  • Fluoroimmunoassay / methods*
  • Humans
  • Immunoglobulin A / analysis
  • Immunoglobulin G / analysis
  • Norwalk virus / immunology*
  • Serologic Tests
  • Time Factors


  • Antibodies, Viral
  • Immunoglobulin A
  • Immunoglobulin G