Anti-aging effects of phloridzin, an apple polyphenol, on yeast via the SOD and Sir2 genes

Biosci Biotechnol Biochem. 2011;75(5):854-8. doi: 10.1271/bbb.100774. Epub 2011 May 20.


The anti-aging effects of phloridzin on the yeast Saccharomyces cerevisiae were investigated by employing a replicative lifespan assay of the K6001 yeast strain. After administrating phloridzin at doses of 3, 10, and 30 µM, the lifespan of the yeast was significantly prolonged in comparison with the untreated group (p<0.01, p<0.001). To determine the mechanism of action, anti-oxidative experiments and ROS assay were performed. Phloridzin improved the viability of the yeast dose-dependently under oxidative stress by 7.5 mM H(2)O(2), and a low dose of phloridzin inhibited ROS of the yeast. Further, SOD1, SOD2, and Sir2 gene expression was examined by reverse transcription-polymerase chain reaction (RT-PCR), and was found to be significantly increased. Finally, superoxide dismutase (SOD) and SIRT1 activity assays showed that phloridzin notably increased the activity of SOD and SIRT1. These results suggest that SOD and Sir2 have important roles in phloridzin-regulated lifespan extension of yeast, and potentially anti-aging effects for mammalian cells via SIRT1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Flavonoids / pharmacology*
  • Gene Expression Regulation, Fungal / drug effects
  • Humans
  • Malus / chemistry*
  • Oxidative Stress / drug effects
  • Phenols / pharmacology*
  • Phlorhizin / pharmacology*
  • Polyphenols
  • Reactive Oxygen Species / metabolism
  • Saccharomyces cerevisiae / drug effects*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism
  • Silent Information Regulator Proteins, Saccharomyces cerevisiae / genetics*
  • Sirtuin 2 / genetics*
  • Superoxide Dismutase / genetics*
  • Superoxide Dismutase / metabolism
  • Time Factors


  • Flavonoids
  • Phenols
  • Polyphenols
  • Reactive Oxygen Species
  • Silent Information Regulator Proteins, Saccharomyces cerevisiae
  • Phlorhizin
  • Superoxide Dismutase
  • SIR2 protein, S cerevisiae
  • Sirtuin 2