The Effect of Compressive Deformations on the Rate of Build-Up of Oxygen in Isolated Skeletal Muscle Cells

Med Eng Phys. 2011 Nov;33(9):1072-8. doi: 10.1016/j.medengphy.2011.04.008. Epub 2011 May 19.

Abstract

In this study we integrated between confocal-based cell-specific finite element (FE) modeling and Virtual Cell (VC) transport simulations in order to determine trends of relationship between externally applied compressive deformations and build-up rates of oxygen in myoblast cells, and to further test how mild culture temperature drops (~3°C) might affect such trends. Geometries of two different cells were used, and each FE cell model was computationally subjected to large compressive deformations. Build-up of oxygen concentrations within the deformed cell shapes over time were calculated using the VC software. We found that the build-up of oxygen in the cells was slightly but consistently hindered when compressive cell deformations were applied. Temperature drops characteristic to ischemic conditions further hinder the oxygen built-up in cells. In a real-world condition, a combination of the deformation and temperature factors should be anticipated, and their combined effect might substantially impair cell respiration functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomechanical Phenomena
  • Cell Line
  • Diffusion
  • Finite Element Analysis
  • Kinetics
  • Mechanical Phenomena*
  • Mice
  • Models, Biological
  • Muscle, Skeletal / cytology*
  • Muscle, Skeletal / metabolism*
  • Oxygen / metabolism*
  • Temperature

Substances

  • Oxygen