Lentiviral vectors to study stochastic noise in gene expression

Methods Enzymol. 2011;497:603-22. doi: 10.1016/B978-0-12-385075-1.00026-3.

Abstract

Lentiviral vectors are vehicles for gene delivery that were originally derived from the human immunodeficiency virus type-1 (HIV-1) lentivirus. These vectors are defective for replication, and thus considered relatively safe, but are capable of stably integrating into the genomic DNA of a broad range of dividing and nondividing mammalian cell types. The ability to stably integrate at semi-random genomic positions make lentiviral vectors a unique and ideal tool for studying stochastic variation in gene expression. Here, we describe the experimental and mathematical methods for using lentiviral vectors to study stochastic noise in gene expression.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Gene Expression*
  • Gene Transfer Techniques
  • Genetic Vectors*
  • HIV-1 / genetics
  • HIV-1 / ultrastructure
  • Humans
  • Lentivirus / genetics*
  • Lentivirus / metabolism
  • Promoter Regions, Genetic
  • Stochastic Processes*
  • Transduction, Genetic