Magnetic separation of cells from developing embryoid bodies using magnetite cationic liposomes

Masanobu Horie; Akira Ito; Takeshi Maki; Yoshinori Kawabe; Masamichi Kamihira

doi:10.1016/j.jbiosc.2011.04.011

Magnetic separation of cells from developing embryoid bodies using magnetite cationic liposomes

J Biosci Bioeng. 2011 Aug;112(2):184-7. doi: 10.1016/j.jbiosc.2011.04.011. Epub 2011 May 23.

Authors

Masanobu Horie¹, Akira Ito, Takeshi Maki, Yoshinori Kawabe, Masamichi Kamihira

Affiliation

¹ Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.

PMID: 21601515
DOI: 10.1016/j.jbiosc.2011.04.011

Abstract

Embryoid bodies resemble post-implantation egg-cylinder stage embryos and are used to differentiate embryonic stem cells in vitro. In this study, we enriched mouse vasa homolog-positive germ cells from embryoid bodies after 8d of differentiation using a magnetic separation method with magnetite cationic liposomes.

MeSH terms

Animals
Cations
Cell Differentiation
Cell Line
Cell Separation / methods*
Cells, Cultured
Embryoid Bodies / cytology*
Embryonic Stem Cells / cytology*
Ferrosoferric Oxide / chemistry*
Germ Cells / cytology
Liposomes / chemistry*
Mice
Mice, Inbred BALB C

Substances

Cations
Liposomes
Ferrosoferric Oxide