Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2011 Jul;193(14):3446-52.
doi: 10.1128/JB.00179-11. Epub 2011 May 20.

Comparative Genomics of the Dormancy Regulons in Mycobacteria

Affiliations
Free PMC article
Comparative Study

Comparative Genomics of the Dormancy Regulons in Mycobacteria

Anna Gerasimova et al. J Bacteriol. .
Free PMC article

Abstract

In response to stresses, Mycobacterium cells become dormant. This process is regulated by the DosR transcription factor. In Mycobacterium tuberculosis, the dormancy regulon is well characterized and contains the dosR gene itself and dosS and dosT genes encoding DosR kinases, nitroreductases (acg; Rv3131), diacylglycerol acyltransferase (DGAT) (Rv3130c), and many universal stress proteins (USPs). In this study, we apply comparative genomic analysis to characterize the DosR regulons in nine Mycobacterium genomes, Rhodococcus sp. RHA1, Nocardia farcinica, and Saccharopolyspora erythraea. The regulons are highly labile, containing eight core gene groups (regulators, kinases, USPs, DGATs, nitroreductases, ferredoxins, heat shock proteins, and the orthologs of the predicted kinase [Rv2004c] from M. tuberculosis) and 10 additional genes with more restricted taxonomic distribution that are mostly involved in anaerobic respiration. The largest regulon is observed in M. marinum and the smallest in M. abscessus. Analysis of large gene families encoding USPs, nitroreductases, and DGATs demonstrates a mosaic distribution of regulated and nonregulated members, suggesting frequent acquisition and loss of DosR-binding sites.

Figures

Fig. 1.
Fig. 1.
Logo of the DosR-binding motif, based on 36 sites (see Table S1 in the supplemental material), as described in Materials and Methods.
Fig. 2.
Fig. 2.
Phylogenetic tree of the DosR and DosS homologs. Regulators (right-hand tree) and kinases (left-hand tree) whose genes are adjacent and likely form operons are linked by dashed lines. Pairs of regulators and kinases in the same locus but not immediately adjacent are linked by dashed lines.

Similar articles

See all similar articles

Cited by 16 articles

See all "Cited by" articles

Publication types

Substances

LinkOut - more resources

Feedback