Effects of 2-deoxyglucose and dehydroepiandrosterone on intracellular NAD(+) level, SIRT1 activity and replicative lifespan of human Hs68 cells

Biogerontology. 2011 Dec;12(6):527-36. doi: 10.1007/s10522-011-9342-7. Epub 2011 May 21.


2-Deoxy-D-glucose (2-DG) and dehydroepiandrosterone (DHEA) have been hypothesized to extend lifespan via mimicking calorie restriction (CR). Activation of sirtuins has been proposed to contribute to life extension of CR by increasing intercellular levels of NAD(+) in several organisms. However, it is unclear whether 2-DG and DHEA may affect intracellular NAD(+) levels and human sirtuin 1 (SIRT1) activities. Here, using human fibroblast Hs68 cells we showed that 2-DG increased intracellular NAD(+) levels in both time- and concentration-dependent manners. 2-DG also dose-dependently increased SIRT1 activities and the lifespan (measured as the cumulated growth curve of population doubling levels) of Hs68 cells. In contrast, DHEA at non-cytotoxic concentrations (≤50 μM) did not significantly affect NAD(+) levels, SIRT1 activities or the lifespan of Hs68 cells. These results suggest that 2-DG extends the lifespan of Hs68 cells by increased NAD(+) levels and SIRT1 activities, and that 2-DG has a potential as a CR mimetic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Caloric Restriction*
  • Cell Line
  • Cell Proliferation / drug effects*
  • Cell Survival / drug effects
  • Cellular Senescence / drug effects*
  • Dehydroepiandrosterone / pharmacology*
  • Deoxyglucose / pharmacology*
  • Dose-Response Relationship, Drug
  • Fibroblasts / drug effects*
  • Fibroblasts / enzymology
  • Fluorometry
  • Humans
  • NAD / metabolism*
  • Sirtuin 1 / metabolism*
  • Time Factors
  • Up-Regulation


  • NAD
  • Dehydroepiandrosterone
  • Deoxyglucose
  • SIRT1 protein, human
  • Sirtuin 1