Tn3 Transposition Immunity Is Conferred by the Transposase-Binding Domain in the Terminal Inverted-Repeat Sequence of Tn3

Gene. 1990 Mar 30;88(1):21-4. doi: 10.1016/0378-1119(90)90055-v.

Abstract

A series of mutant terminal inverted repeats (IRs), having 2 bp substitutions at various sites within the 38-bp IR sequence of the ampicillin-resistance transposon Tn3, were tested for transposition immunity to Tn3. Mutations within region 1-10 in the IR did not affect transposition immunity, while mutations within region 13-38 inactivated the immunity function. These two regions corresponded to domain A which was not bound specifically by Tn3 transposase and to domain B which was bound by the transposase, respectively. This indicates that specific binding of transposase to domain B within the IR sequence is responsible for transposition immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ampicillin Resistance*
  • Base Sequence
  • Chromosome Inversion*
  • DNA Transposable Elements*
  • Molecular Sequence Data
  • Mutation
  • Nucleotidyltransferases / metabolism*
  • Plasmids
  • Repetitive Sequences, Nucleic Acid*
  • Transposases

Substances

  • DNA Transposable Elements
  • Nucleotidyltransferases
  • Transposases