Multiplex PCR as a tool for validating plasmid content of Borrelia burgdorferi

J Microbiol Methods. 2011 Aug;86(2):243-7. doi: 10.1016/j.mimet.2011.05.004. Epub 2011 May 12.

Abstract

Borrelia burgdorferi has an unusual genomic structure containing 21 plasmids. These plasmids carry genes that are essential for infectivity and survival of the spirochetes in vivo. Several plasmids are lost during cultivation in vitro, which might lead to a heterogeneous population after multiple passages and loss of infectivity in laboratory animals. Herein, we present a simple and inexpensive multiplex PCR method that detects the complete plasmid profile of B. burgdorferi B31 in just two PCR tubes.

MeSH terms

  • Borrelia burgdorferi / genetics*
  • DNA, Bacterial / genetics
  • Electrophoresis, Agar Gel
  • Molecular Biology / methods*
  • Plasmids / analysis*
  • Polymerase Chain Reaction / methods*

Substances

  • DNA, Bacterial