Anti-inflammatory properties from isolated compounds of Cyclolepis genistoides

Pharm Biol. 2011 Jul;49(7):675-8. doi: 10.3109/13880200903431467. Epub 2011 May 25.


Context: Cyclolepis genistoides D. Don (Asteraceae) is an Argentinean endemic shrub, known by the vernacular name "palo azul" or "matorro negro". It is widely used in folk medicine as a diuretic, an antirheumatic, and an antispasmodic agent.

Objective: The aim of this work was to describe the activity of two isolated compounds of C. genistoides, oleanolic acid (1) and deacylcynaropicrin (2), using the carrageenan-induced paw edema method in mice.

Materials and methods: Aerial parts of C. genistoides were dried, powdered, and extracted with petroleum ether, ethyl acetate, dichloromethane, and methanol for 48 h. The fractions obtained from the ethyl acetate extract yielded oleanolic acid, while deacylcynaropicrin was obtained according to Abdei-Mogib et al. Structures were elucidated by ¹H-NMR and ¹³C-NMR. The products were administered intraperitoneally at doses of 40, 75, and 100 mg/kg.

Results and discussion: Compound 1 exhibited significant activity during the first 7 h of the inflammatory phase (at 1, 3, 5, and 7 h), exercising its inhibitory action on inflammation mediated by histamine, prostaglandins, serotonin, and kinins, while compound 2 showed a significant inhibition at 3 and 5 h contributing to this effect, acting in the intermediate phase.

Conclusions: According to the results of this work, the intraperitoneal administration of oleanolic acid and deacylcynaropicrin isolated from the aerial parts of C. genistoides produced a significant inhibition of carrageenan-induced inflammation at doses of 75 and 100 mg/kg. These results give support to the use of this plant as an anti-inflammatory in traditional medicine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology*
  • Asteraceae / chemistry*
  • Carrageenan
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Female
  • Inflammation / drug therapy
  • Inflammation / physiopathology
  • Injections, Intraperitoneal
  • Lactones / administration & dosage
  • Lactones / isolation & purification
  • Lactones / pharmacology*
  • Male
  • Medicine, Traditional
  • Mice
  • Oleanolic Acid / administration & dosage
  • Oleanolic Acid / isolation & purification
  • Oleanolic Acid / pharmacology*
  • Plant Components, Aerial
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology
  • Sesquiterpenes / administration & dosage
  • Sesquiterpenes / isolation & purification
  • Sesquiterpenes / pharmacology*
  • Time Factors


  • Anti-Inflammatory Agents
  • Lactones
  • Plant Extracts
  • Sesquiterpenes
  • deacylcynaropicrin
  • Oleanolic Acid
  • Carrageenan