Synthesis and use of a novel N-formyl peptide derivative to isolate a human N-formyl peptide receptor cDNA

Biochem Biophys Res Commun. 1990 May 16;168(3):1103-9. doi: 10.1016/0006-291x(90)91143-g.


N-formyl-methionyl peptides are powerful chemoattractants which bind to specific receptors on the neutrophil plasma membrane. A cDNA library from HL-60 cells, differentiated into granulocytes highly responsive to N-formyl-methionyl peptides, was constructed in the COS cell expression vector CDM8. A cDNA clone was isolated that conferred to COS cells the ability to bind a new and highly efficient hydrophilic derivative of N-formyl-Met-Leu-Phe-Lys. The transfected COS cells displayed two classes of binding sites with Kd values of 0.5-1 nM and 5-10 nM, respectively. The cDNA was 1.9 kb long with a 1050 bp open reading frame encoding a 350 residue protein. The hydropathy plot analysis revealed seven hydrophobic segments, a pattern quite similar to that of G protein-coupled receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA / isolation & purification*
  • Gene Library
  • Humans
  • Molecular Sequence Data
  • N-Formylmethionine Leucyl-Phenylalanine / analogs & derivatives*
  • N-Formylmethionine Leucyl-Phenylalanine / metabolism
  • Receptors, Formyl Peptide
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / metabolism
  • Superoxide Dismutase / biosynthesis
  • Transfection


  • Receptors, Formyl Peptide
  • Receptors, Immunologic
  • N-Formylmethionine Leucyl-Phenylalanine
  • DNA
  • Superoxide Dismutase

Associated data

  • GENBANK/M37128