A novel promising therapy for vein graft restenosis: overexpressed Nogo-B induces vascular smooth muscle cell apoptosis by activation of the JNK/p38 MAPK signaling pathway

Med Hypotheses. 2011 Aug;77(2):278-81. doi: 10.1016/j.mehy.2011.04.035. Epub 2011 May 25.

Abstract

Coronary artery bypass grafting using autologous saphenous veins is a standard surgical therapy for coronary artery diseases. However, post-procedure vein graft restenosis impedes its effectiveness and often leads to a high morbidity and mortality, and a reduction in the quality of life. Neointimal hyperplasia is a major cause of vein graft occlusion, and is characterized by an imbalance between vascular smooth muscle cell (VSMC) proliferation and apoptosis. So far, there have been no optimally effective pharmacological or non-pharmacological interventions for the prevention and treatment of vein graft occlusion. Gene therapy has emerged as a potential therapeutic approach, as bypass grafts can be genetically modified ex vivo prior to grafting in the coronary vasculature. Nogo-B, recognized as a vascular protective factor, has been shown to reduce neointimal thickening in graft veins, but its specific mechanism is uncertain. Evidence from diverse sources has documented that overexpressed Nogo-B can induce apoptosis of variant cancer cell lines, suggesting that overexpressed Nogo-B may have a pro-apoptotic role in VSMC. Furthermore, we have found that Nogo-B is associated with the mitogen-activated protein kinase (MAPK) signaling pathway, which plays important roles in cell growth, differentiation, and apoptosis. Recent studies have shown that VSMC apoptosis can be induced by activation of the c-Jun-N-terminal kinase (JNK)/p38 MAPK pathway. Therefore, we propose that overproduction of Nogo-B in the graft vein could result in reduced neointimal hyperplasia, the mechanism of which involves increased VSMC apoptosis induced by activation of the JNK/p38 MAPK pathway. This study will provide a new clue for gene therapy in the treatment of vein graft failure.

MeSH terms

  • Apoptosis / genetics
  • Apoptosis / physiology*
  • Coronary Artery Bypass / adverse effects*
  • Enzyme Activation / genetics
  • Enzyme Activation / physiology*
  • Genetic Therapy / methods*
  • Graft Occlusion, Vascular / drug therapy*
  • Graft Occlusion, Vascular / genetics
  • Humans
  • MAP Kinase Kinase 4 / metabolism
  • Models, Biological
  • Myelin Proteins / genetics
  • Myelin Proteins / metabolism*
  • Myocytes, Smooth Muscle / metabolism*
  • Nogo Proteins
  • Signal Transduction / physiology*
  • p38 Mitogen-Activated Protein Kinases / metabolism

Substances

  • Myelin Proteins
  • Nogo Proteins
  • RTN4 protein, human
  • p38 Mitogen-Activated Protein Kinases
  • MAP Kinase Kinase 4