Micrometric molecular histology of lipids by mass spectrometry imaging

Curr Opin Chem Biol. 2011 Oct;15(5):725-32. doi: 10.1016/j.cbpa.2011.04.017. Epub 2011 May 24.

Abstract

Time-Of-Flight Secondary Ion Mass Spectrometry is compared to other mass spectrometry imaging techniques, and recent improvements of the experimental methods, driven by biological and biomedical applications, are described and discussed. This review shows that this method that can be considered as a micrometric molecular histology is particularly efficient for obtaining images of various lipid species at the surface of a tissue sample, without sample preparation, and with a routine spatial resolution of 1μm or less.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Histological Techniques*
  • Humans
  • Lipids / analysis*
  • Mice
  • Molecular Imaging / instrumentation
  • Molecular Imaging / methods*
  • Signal Transduction
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • Spectrometry, Mass, Secondary Ion / instrumentation
  • Spectrometry, Mass, Secondary Ion / methods*
  • Spectroscopy, Fourier Transform Infrared

Substances

  • Lipids