Plasma rich in growth factors (PRGF-Endoret) stimulates proliferation and migration of primary keratocytes and conjunctival fibroblasts and inhibits and reverts TGF-beta1-Induced myodifferentiation

Invest Ophthalmol Vis Sci. 2011 Aug 1;52(9):6066-73. doi: 10.1167/iovs.11-7302.


Purpose: Plasma rich in growth factors (PRGF-Endoret) technology is an autologous platelet-enriched plasma obtained from patient's own blood, which after activation with calcium chloride allows the release of a pool of biologically active proteins that influence and promote a range of biological processes including cell recruitment, and growth and differentiation. Because ocular surface wound healing is mediated by different growth factors, we decided to explore the potential of PRGF-Endoret technology in stimulating the biological processes related with fibroblast-induced tissue repair. Furthermore, the anti-fibrotic properties of this technology were also studied.

Methods: Blood from healthy donors was collected, centrifuged and, whole plasma column (WP) and the plasma fraction with the highest platelet concentration (F3) were drawn off, avoiding the buffy coat. Primary human cells including keratocytes and conjunctival fibroblasts were used to perform the "in vitro" investigations. The potential of PRGF-Endoret in promoting wound healing was evaluated by means of a proliferation and migration assays. Fibroblast cells were induced to myofibroblast differentiation after the treatment with 2.5 ng/mL of TGF-β1. The capability of WP and F3 to prevent and inhibit TGF-β1-induced differentiation was evaluated.

Results: Results show that this autologous approach significantly enhances proliferation and migration of both keratocytes and conjunctival fibroblasts. In addition, plasma rich in growth factors prevents and inhibits TGF-β1-induced myofibroblast differentiation. No differences were found between WP and F3 plasma fractions.

Conclusions: These results suggest that PRGF-Endoret could reduce scarring while stimulating wound healing in ocular surface. F3 or whole plasma column show similar biological effects in keratocytes and conjunctival fibroblast cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Donors
  • Cell Differentiation / drug effects
  • Cell Movement / drug effects*
  • Cell Proliferation / drug effects*
  • Cells, Cultured
  • Conjunctiva / cytology*
  • Cornea / cytology*
  • Enzyme-Linked Immunosorbent Assay
  • Fibroblasts / cytology*
  • Humans
  • Intercellular Signaling Peptides and Proteins / pharmacology
  • Male
  • Myofibroblasts / drug effects
  • Platelet-Rich Plasma / physiology*
  • Transforming Growth Factor beta1 / pharmacology*


  • Intercellular Signaling Peptides and Proteins
  • Transforming Growth Factor beta1