OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis

Plant J. 2011 Aug;67(4):583-94. doi: 10.1111/j.1365-313X.2011.04615.x. Epub 2011 May 25.

Abstract

Shugoshin is a conserved protein in eukaryotes that protects the centromeric cohesin of sister chromatids from cleavage by separase during meiosis. In this study, we identify the rice (Oryza sativa, 2n=2x=24) homolog of ZmSGO1 in maize (Zea mays), named OsSGO1. During both mitosis and meiosis, OsSGO1 is recruited from nucleoli onto centromeres at the onset of prophase. In the Tos17-insertional Ossgo1-1 mutant, centromeres of sister chromatids separate precociously from each other from metaphase I, which causes unequal chromosome segregation during meiosis II. Moreover, the release of OsSGO1 from nucleoli is completely blocked in Ossgo1-1, which leads to the absence of OsSGO1 in centromeric regions after the onset of mitosis and meiosis. Furthermore, the timely assembly and maintenance of synaptonemal complexes during early prophase I are affected in Ossgo1 mutants. Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • Centromere / metabolism*
  • Chromatids / metabolism*
  • Chromosome Segregation / genetics
  • Chromosomes, Plant / genetics
  • DNA, Complementary / genetics
  • Flowers / genetics
  • Flowers / metabolism
  • Flowers / ultrastructure
  • Meiosis / genetics*
  • Mitosis / genetics
  • Mutagenesis, Insertional
  • Oryza / genetics
  • Oryza / metabolism*
  • Oryza / ultrastructure
  • Plant Leaves / genetics
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plant Roots / genetics
  • Plant Roots / metabolism
  • Plant Roots / ultrastructure
  • Plant Stems / genetics
  • Protein Transport
  • RNA Interference
  • RNA, Plant / genetics
  • Synaptonemal Complex / metabolism*

Substances

  • Cell Cycle Proteins
  • DNA, Complementary
  • Plant Proteins
  • RNA, Plant