Cloning and antibody recognition analysis of the canine 5-lipoxygenase gene

Vet Immunol Immunopathol. 2011 Aug 15;142(3-4):276-9. doi: 10.1016/j.vetimm.2011.05.006. Epub 2011 May 7.


5-Lipoxygenase cDNA was prepared from canine white blood cells revealing the full-length message using an oligonucleotide capping method. The sequenced 5-Lipoxygenase open reading frame revealed a 2031 base pair message encoding a 676 amino acid protein. The amino acid sequence showed mild variation with the presumed canine sequence, as well as differences in important residues of known phosphorylation observed in other species. The sequence had between 86 and 92% homology with other species, revealing a highly conserved sequence. Confirmation of gene product identity was achieved through transient transfection of the gene in a V5-Histidine tagged pcDNA 3.1 vector into a known canine cell line. Both V5 antibody and 5-lipoxygenase antibody confirmed the gene product using Western blotting and immunoflourescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arachidonate 5-Lipoxygenase / genetics*
  • Arachidonate 5-Lipoxygenase / immunology
  • Base Sequence
  • Blotting, Western / veterinary
  • Cell Line, Tumor
  • Cloning, Molecular
  • Dogs
  • Molecular Sequence Data
  • RNA / chemistry
  • RNA / genetics
  • Reverse Transcriptase Polymerase Chain Reaction / veterinary
  • Sequence Alignment
  • Transfection / veterinary


  • RNA
  • Arachidonate 5-Lipoxygenase

Associated data

  • GENBANK/HQ657323