E-cadherin Is Crucial for Embryonic Stem Cell Pluripotency and Can Replace OCT4 During Somatic Cell Reprogramming

EMBO Rep. 2011 Jul 1;12(7):720-6. doi: 10.1038/embor.2011.88.

Abstract

We report new functions of the cell-adhesion molecule E-cadherin in murine pluripotent cells. E-cadherin is highly expressed in mouse embryonic stem cells, and interference with E-cadherin causes differentiation. During cellular reprogramming of mouse fibroblasts by OCT4, SOX2, KLF4 and c-MYC, fully reprogrammed cells were exclusively observed in the E-cadherin-positive cell population and could not be obtained in the absence of E-cadherin. Moreover, reprogrammed cells could be established by viral E-cadherin in the absence of exogenous OCT4. Thus, reprogramming requires spatial cues that cross-talk with essential transcription factors. The cell-adhesion molecule E-cadherin has important functions in pluripotency and reprogramming.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cadherins / genetics
  • Cadherins / metabolism*
  • Cell Differentiation / genetics
  • Cell Line
  • Cellular Reprogramming / genetics*
  • Embryonic Stem Cells / metabolism*
  • Gene Deletion
  • Gene Expression Regulation, Developmental
  • Mice
  • Mice, 129 Strain
  • Mice, Inbred NOD
  • Mice, SCID
  • Octamer Transcription Factor-3 / genetics
  • Octamer Transcription Factor-3 / metabolism*
  • Pluripotent Stem Cells / cytology
  • Pluripotent Stem Cells / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • Cadherins
  • Octamer Transcription Factor-3
  • Pou5f1 protein, mouse
  • RNA, Messenger