Relationship between lunasin's sequence and its inhibitory activity of histones H3 and H4 acetylation

Mol Nutr Food Res. 2011 Jul;55(7):989-98. doi: 10.1002/mnfr.201000632. Epub 2011 May 25.

Abstract

Scope: Dysfunction of histone acetyltransferases (HATs) or histone deacetylases (HDACs) involved in histones acetylation has been associated with cancer. Inhibitors of these enzymes are becoming potential targets for new therapies.

Methods and results: This study reports by Western-Blot analysis, that peptide lunasin is mainly an in vitro inhibitor of histone H4 acetylation by P300/cAMP-response element-binding protein (CBP)-associated factor (PCAF), with IC₅₀ values dependent on the lysine position sensitive to be acetylated (0.83 μM (H4-Lys 8), 1.27 μM (H4-Lys 12) and 0.40 μM (H4-Lys 5, 8, 12, 16)). Lunasin is also capable of inhibiting H3 acetylation (IC₅₀ of 5.91 μM (H3-Lys 9) and 7.81 μM (H3-Lys 9, 14)). Studies on structure-activity relationship establish that lunasin's sequence are essential for inhibiting H4 acetylation whereas poly-D sequence is the main active sequence responsible for H3 acetylation inhibition. Lunasin also inhibits H3 and H4 acetylation and cell proliferation (IC₅₀ of 181 μM) in breast cancer MDA-MB-231 cells. Moreover, this peptide decreases expression of cyclins and cyclin dependent kinases-4 and -6, implicated in cell cycle pathways.

Conclusion: Results from this study demonstrates lunasin's role as modulator of histone acetylation and protein expression that might contribute on its chemopreventive properties against breast cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation / drug effects
  • Amino Acid Sequence
  • Biomarkers / metabolism
  • Breast Neoplasms / drug therapy
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • Cell Cycle / drug effects
  • Cell Cycle / physiology
  • Cell Line, Tumor
  • Cell Proliferation
  • Cyclin-Dependent Kinase 4 / drug effects
  • Cyclin-Dependent Kinase 4 / metabolism
  • Cyclin-Dependent Kinase 6 / drug effects
  • Cyclin-Dependent Kinase 6 / metabolism
  • Cyclins / drug effects
  • Cyclins / metabolism
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology
  • Histone Acetyltransferases / antagonists & inhibitors*
  • Histones / drug effects
  • Histones / metabolism*
  • Humans
  • Inhibitory Concentration 50
  • Lysine / metabolism
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Peptide Fragments / pharmacology
  • Soybean Proteins / chemistry*
  • Soybean Proteins / pharmacology*
  • Structure-Activity Relationship
  • p300-CBP Transcription Factors / antagonists & inhibitors

Substances

  • Biomarkers
  • Cyclins
  • Enzyme Inhibitors
  • GM2S-1 protein, Glycine max
  • Histones
  • Peptide Fragments
  • Soybean Proteins
  • Histone Acetyltransferases
  • p300-CBP Transcription Factors
  • p300-CBP-associated factor
  • CDK4 protein, human
  • CDK6 protein, human
  • Cyclin-Dependent Kinase 4
  • Cyclin-Dependent Kinase 6
  • Lysine