Detection of integrin-linked kinase in the serum of patients with malignant pleural mesothelioma

J Thorac Cardiovasc Surg. 2011 Aug;142(2):384-9. doi: 10.1016/j.jtcvs.2011.04.034. Epub 2011 May 26.

Abstract

Objective: Integrin-linked kinase, which is relevant to neoplastic transformation, is highly expressed in malignant pleural mesothelioma. Recently, detection of integrin-linked kinase in serum of patients with ovarian cancer has been reported. This study asks whether integrin-linked kinase can also be detected in serum of patients with malignant pleural mesothelioma and whether serum level has diagnostic or prognostic relevance for that disease.

Methods: A sandwich enzyme-linked immunosorbent assay was designed to detect integrin-linked kinase and applied to serum samples from 46 patients with malignant pleural mesothelioma, 98 patients with other malignant chest disease, and 23 patients with benign chest disease. Integrin-linked kinase serum concentration and clinical data were correlated statistically.

Results: Median serum integrin-linked kinase concentration was significantly higher in malignant pleural mesothelioma (8.89 ng/mL) than in other malignant chest disease (0.66 ng/mL) or benign chest disease (0.78 ng/mL, P < .001). There was no relevant correlation of serum integrin-linked kinase with cell lysis parameters (R(2) < 0.1). Serum integrin-linked kinase concentration greater than 2.48 ng/mL had diagnostic sensitivity of 80%, specificity of 95%, positive predictive value of 85.7%, negative predictive value of 92.7%, and overall accuracy of 91% for distinction between malignant pleural mesothelioma and other diseases. Serum integrin-linked kinase concentration in malignant pleural mesothelioma was independent of histologic subtype or asbestos exposure. There was no statistically significant impact of serum integrin-linked kinase concentration on prognosis.

Conclusions: Integrin-linked kinase can be detected in serum of patients with malignant pleural mesothelioma and may be a diagnostic marker for the disease.

MeSH terms

  • Aged
  • Biomarkers / blood
  • Cell Transformation, Neoplastic
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Male
  • Mesothelioma / blood*
  • Mesothelioma / diagnosis
  • Mesothelioma / enzymology
  • Middle Aged
  • Pleural Neoplasms / blood*
  • Pleural Neoplasms / diagnosis
  • Pleural Neoplasms / enzymology
  • Prognosis
  • Protein-Serine-Threonine Kinases / blood*
  • Sensitivity and Specificity

Substances

  • Biomarkers
  • integrin-linked kinase
  • Protein-Serine-Threonine Kinases