Cellular mechanisms in the superinfection exclusion of vesicular stomatitis virus

Virology. 1990 Jul;177(1):375-9. doi: 10.1016/0042-6822(90)90494-c.


The superinfection exclusion of VSV has been studied and found to be caused by a combination of three distinct effects on endocytosis by VSV-infected cells: first, a decreased rate of formation of endocytic vesicles as judged by an inhibition of fluid-phase uptake at 2 hr postinfection; second, a decreased rate of internalization of receptor-bound ligands, which was detected at 4 hr postinfection; and third, a competition with newly synthesized virus for occupancy of coated pits, as indicated by electron microscopy of infected cells. At the same time that fluid-phase uptake decreased, numerous uncoated invaginations were observed at the cell surface.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Cell Membrane / ultrastructure*
  • Cell Transformation, Viral*
  • Coated Pits, Cell-Membrane / ultrastructure
  • Cricetinae
  • Endocytosis
  • Kinetics
  • Microscopy, Electron
  • Superinfection
  • Vesicular stomatitis Indiana virus / pathogenicity*
  • Vesicular stomatitis Indiana virus / physiology