Developmental patterns of PPAR and RXR gene expression during spermatogenesis

Front Biosci (Elite Ed). 2011 Jun 1;3:1209-20.

Abstract

Members of the family of nuclear receptors that include peroxisome proliferator-activated receptors (PPARs) and retinoid X receptors (RXRs) are important mediators of selective gene activation during development and cellular differentiation. In this study, developmentally-specific PPAR and RXR patterns of expression that occur in somatic and germ cell populations in the testis were determined using quantative real-time PCR (qRT-PCR) studies on RNAs that were isolated from StaPut purified mouse germ cells and primary rat Sertoli cells. These qRT-PCR studies indicate that transcripts encoding the PPAR-Alpha (α), -Beta (β), and -Gamma (γ) and RXR -Alpha (α), -Beta (β), and -Gamma (γ) are developmentally expressed in both differentiating germ and Sertoli cells. In further experiments aimed at deciphering the physiological role that PPAR-Gamma (γ) plays in Sertoli cells, 15-day primary rat Sertoli cells were infected with recombinant adenoviral vectors containing PPAR-Gamma (γ) cDNA and PPAR-Gamma (γ) RNAi constructs. Affymetrix microarray analysis and qRT-PCR validation studies using total RNA isolated from these transfected cells indicated that PPAR-Gamma regulates the pattern of expression of key lipid metabolic genes in Sertoli cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Base Sequence
  • Cells, Cultured
  • DNA Primers
  • Gene Expression Regulation, Developmental*
  • Male
  • Mice
  • Oligonucleotide Array Sequence Analysis
  • Peroxisome Proliferator-Activated Receptors / genetics*
  • Polymerase Chain Reaction
  • Rats
  • Rats, Sprague-Dawley
  • Retinoid X Receptors / genetics*
  • Spermatogenesis / genetics*

Substances

  • DNA Primers
  • Peroxisome Proliferator-Activated Receptors
  • Retinoid X Receptors