Involvement of LOC66273 isoform 2, a novel Mth938 containing protein, in MAPK pathway

Front Biosci (Elite Ed). 2011 Jun 1;3:1241-8.

Abstract

Using dual-luciferase reporter assay system, our previous study showed that LI2, significantly increased AP-1 transcriptional activity. Sub-cellular localization showed that GFP-LI2 fusion protein is diffusely distributed in the cytoplasm, with some highly concentrated spots around the nucleus, suggesting that LI2 protein has a physiological role in cytoplasm. Overexpression of LI2 significantly increased AP-1 transcriptional activity. Moreover, LI2 significantly promoted transcriptional activity of Elk1 and c-jun, which might, at least partly, be associated with activated ERK1/2 and JNK/SAPK signaling pathway. These data suggest that LI2 is a novel MAPK regulating protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • DNA Primers
  • DNA, Complementary
  • Gene Expression Profiling
  • Humans
  • MAP Kinase Signaling System*
  • Mice
  • Molecular Sequence Data
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • DNA Primers
  • DNA, Complementary