Interactions of Thyroid Hormone and FSH in the Regulation of Rat Granulosa Cell Apoptosis

Front Biosci (Elite Ed). 2011 Jun 1;3:1401-13.


Thyroid hormone (TH) is important for normal reproductive function. Our previous studies indicate that FSH increases preantral follicle growth in vitro, a response markedly enhanced by triiodothyronine (T3). However, the nature of this hormonal interaction is poorly understood. The objective of this study was to determine if and how T3 modulate FSH-induced expression and actions of granulosa cell intracellular survival and death intermediates. We investigated the possible involvement of Src and PI3K/Akt pathway in the regulation of granulosa cell survival. We demonstrated that, while ineffective alone (0.1-100 nM), T3 markedly enhanced FSH (100 ng/ml)-induced granulosa cell phospho-Src and phospho-Akt contents and Xiap expression in vitro. The effects of T3 were concentration-dependent, with maximal responses at 1.0 nM. FSH alone decreased Fas Ligand (FasL) content irrespective of the presence of T3. Co-treatment of cell with T3 and FSH decreased Fas content, although neither hormone alone elicited a significant response. Taken together, the present study demonstrates that T3 potentiates the cell survival action of FSH through Src- and PI3K-mediated Xiap up-regulation and decreased Fas and FasL expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / physiology*
  • Base Sequence
  • Blotting, Western
  • DNA Primers
  • Female
  • Follicle Stimulating Hormone / metabolism*
  • Granulosa Cells / cytology*
  • In Situ Nick-End Labeling
  • Polymerase Chain Reaction
  • Rats
  • Thyroid Hormones / metabolism*


  • DNA Primers
  • Thyroid Hormones
  • Follicle Stimulating Hormone