MicroRNA-regulated transgene expression systems for gene therapy and virotherapy

Front Biosci (Landmark Ed). 2011 Jun 1;16:2389-401. doi: 10.2741/3861.

Abstract

For safe and effective gene therapy, targeted tissue-restricted transgene expression is desirable. Various methods have been developed to achieve such expression, including the use of tissue-specific promoters. In addition to these approaches, a new system which can regulate transgene expression, including viral gene expression, by exploiting microRNAs (miRNAs) has recently been developed. miRNAs are approximately 22-nucleotide (nt)-long non-coding RNAs that translationally suppress or catalytically degrade target mRNA through binding to imperfectly complementary sequences in the 3'-untranslated region (UTR). In miRNA-regulated transgene expression systems, tandem copies of sequences perfectly complementary to the miRNAs are usually incorporated into the 3'-UTR of the transgene expression cassette, leading to the suppression of transgene expression in cells expressing the corresponding miRNAs. miRNA-mediated regulation of transgene expression was first demonstrated for lentivirus vectors, and subsequently this technology was applied to replication-incompetent adenovirus vectors, tumor-specific oncolytic viruses for cancer therapy, and recombinant live attenuated viruses for vaccine therapy. The aim of this review is to highlight the applications of miRNA-regulated transgene expression systems for gene therapy and virotherapy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • 3' Untranslated Regions
  • Adenoviridae / genetics
  • Animals
  • Gene Expression
  • Genes, Transgenic, Suicide
  • Genetic Therapy / methods*
  • Genetic Vectors
  • Humans
  • MicroRNAs / genetics*
  • Oncolytic Virotherapy / methods*
  • RNA Interference
  • Virus Replication

Substances

  • 3' Untranslated Regions
  • MicroRNAs