The vitamin B12 absorption test, CobaSorb, identifies patients not requiring vitamin B12 injection therapy

Scand J Clin Lab Invest. 2011 Sep;71(5):432-8. doi: 10.3109/00365513.2011.581389. Epub 2011 May 30.

Abstract

Background: Treatment with vitamin B12 has virtually no side effects; however, life-long treatment is inconvenient for the patient and constitutes a cost for society.

Objective: To investigate whether vitamin B12 injection treatment reflects the actual need for treatment or whether some patients are treated unnecessarily with vitamin B12 injections.

Material and methods: A prospective intervention study was conducted among nine general practitioners in Western Sealand County, Denmark. Forty-four patients older than 18 years who had received injection therapy with vitamin B12 for a median of eight years (range 1-26 years) were included. After discontinuation of vitamin B12 injections, blood samples were analysed monthly for hemoglobin, cobalamin, holotranscobalamin, homocysteine and methylmalonic acid. The capacity to absorb vitamin B12 was examined after a median of 13 months (range 5-32 months) by measurement of holotranscobalamin or cyanocobalamin on transcobalamin before and after 1 and 2 days intake of 3 × 9 μg of vitamin B12. Patients unable to absorb the vitamin continued treatment with vitamin B12 injection. The remaining patients participated in a follow-up study receiving 9 μg oral vitamin B12 daily or no vitamin B12 substitution.

Results: Of the 44 patients studied, 35 patients were able to absorb vitamin B12. None of the patients included in the follow-up study showed biochemical signs of vitamin B12 deficiency by the end of the study.

Conclusion: Our results suggest that the capacity for absorbing vitamin B12 should be examined prior to the choice of treatment.

Trial registration: ClinicalTrials.gov NCT00326833.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers / blood
  • Blood Chemical Analysis / methods*
  • Female
  • Hemoglobins / metabolism
  • Homocysteine / blood
  • Humans
  • Injections
  • Intestinal Absorption
  • Male
  • Methylmalonic Acid / blood
  • Middle Aged
  • Prospective Studies
  • Reference Values
  • Transcobalamins / chemistry
  • Transcobalamins / metabolism
  • Vitamin B 12 / administration & dosage*
  • Vitamin B 12 / metabolism
  • Vitamin B 12 / therapeutic use
  • Vitamin B 12 Deficiency / blood
  • Vitamin B 12 Deficiency / drug therapy*

Substances

  • Biomarkers
  • Hemoglobins
  • Transcobalamins
  • Homocysteine
  • Methylmalonic Acid
  • Vitamin B 12

Associated data

  • ClinicalTrials.gov/NCT00326833