A luminescent oxygen channeling biosensor that measures small GTPase activation

Chem Commun (Camb). 2011 Jul 14;47(26):7521-3. doi: 10.1039/c1cc11944c. Epub 2011 May 31.

Abstract

We established a homogeneous luminescent oxygen channeling sensor for measuring activation states of small GTPases. The assay quantifies activated GTPases in cell lysates, can be applied to different GTPases, and can be used for multiplex screening. The study will provide guidelines for determining activation states of diverse GTPases in various biological contexts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biosensing Techniques / methods*
  • Enzyme Activation
  • Luminescent Measurements*
  • Mice
  • Monomeric GTP-Binding Proteins / metabolism*
  • NIH 3T3 Cells
  • Oxygen / metabolism*
  • Time Factors

Substances

  • Monomeric GTP-Binding Proteins
  • Oxygen