Sesquarterpenes (C35 terpenes) biosynthesized via the cyclization of a linear C35 isoprenoid by a tetraprenyl-β-curcumene synthase and a tetraprenyl-β-curcumene cyclase: identification of a new terpene cyclase

Tsutomu Sato; Satoru Yoshida; Hiroko Hoshino; Mizuki Tanno; Mami Nakajima; Tsutomu Hoshino

doi:10.1021/ja203779h

Sesquarterpenes (C35 terpenes) biosynthesized via the cyclization of a linear C35 isoprenoid by a tetraprenyl-β-curcumene synthase and a tetraprenyl-β-curcumene cyclase: identification of a new terpene cyclase

J Am Chem Soc. 2011 Jun 29;133(25):9734-7. doi: 10.1021/ja203779h. Epub 2011 Jun 7.

Authors

Tsutomu Sato¹, Satoru Yoshida, Hiroko Hoshino, Mizuki Tanno, Mami Nakajima, Tsutomu Hoshino

Affiliation

¹ Department of Applied Biological Chemistry, Faculty of Agriculture, and Graduate School of Science and Technology, Niigata University, 8050 Ikarashi-2, Niigata 950-2181, Japan. satot@agr.niigata-u.ac.jp

PMID: 21627333
DOI: 10.1021/ja203779h

Abstract

In this study, mono- and pentacyclic C(35) terpenes from Bacillus subtilis were biosynthesized via the cyclization of C(35) isoprenoid using purified enzymes, including the first identified new terpene cyclase that shows no sequence homology to any of the known terpene cyclases. On the basis of these findings, we propose that these C(35) terpenes should be called the new family of "sesquarterpenes."

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / enzymology*
Bacillus subtilis / metabolism
Curcumin / analogs & derivatives
Cyclization
Enzymes / metabolism*
Terpenes* / metabolism

Substances

Enzymes
Terpenes
Curcumin