High spatial resolution imaging mass spectrometry and classical histology on a single tissue section

J Mass Spectrom. 2011 Jun;46(6):568-71. doi: 10.1002/jms.1926.


The work presented in this report describes and demonstrates a protocol for protein imaging analysis of biological tissue using MALDI IMS where histological staining and MS analysis are performed on the same tissue section. Spatial image resolution is shown at 35 µm for sagittal sections of the cerebellum from rat brain.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cerebellum / chemistry
  • Female
  • Histocytochemistry / methods*
  • Histocytological Preparation Techniques
  • Male
  • Molecular Imaging / methods*
  • Nerve Tissue Proteins / analysis
  • Nerve Tissue Proteins / chemistry*
  • Rats
  • Rats, Sprague-Dawley
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*


  • Nerve Tissue Proteins