Virucidal effect of ozone treatment of laboratory animal viruses

Jikken Dobutsu. 1990 Apr;39(2):223-9. doi: 10.1538/expanim1978.39.2_223.


An ozonization method was used to inactivate the viral pathogens of laboratory animals. Ozone at a concentration of over 100 ppm with high humidity was highly virucidal against 4 RNA viruses: HVJ, Theiler's murine encephalomyelitis virus (TMEV), Reo type 3 virus (RV) and murine hepatitis virus (MHV). For the ozone tests, 0.1 ml of a virus suspension in deionized water or saline and was placed in 35-mm dishes. The titer of 10(6) plaque-forming units of TMEV in a liquid-phase, which was highly stable against physical treatments, was reduced within 1 hr to a level of 0 by 300 ppm of ozone at 80% humidity and 22-25 degrees C. HVJ and MHV were more susceptible than TMEV to the ozone treatment. RV was the most resistant of the 4 viruses. The ozonization method may be a good way to disinfect not only for the laboratory animal RNA-viruses (both of enveloped and unenveloped viruses) but also animal rooms, clean rooms and even safety cabinets.

MeSH terms

  • Animals
  • Animals, Laboratory / microbiology*
  • Antiviral Agents*
  • Culture Media
  • Disinfectants
  • Humidity
  • Mammalian orthoreovirus 3 / drug effects
  • Maus Elberfeld virus / drug effects
  • Microbial Sensitivity Tests
  • Murine hepatitis virus / drug effects
  • Ozone / pharmacology*
  • Parainfluenza Virus 1, Human / drug effects
  • Phosphates / pharmacology
  • RNA Viruses / drug effects*


  • Antiviral Agents
  • Culture Media
  • Disinfectants
  • Phosphates
  • Ozone