Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Jun;6(6):743-60.
doi: 10.1038/nprot.2011.319. Epub 2011 May 5.

Web-based Inference of Biological Patterns, Functions and Pathways From Metabolomic Data Using MetaboAnalyst

Affiliations

Web-based Inference of Biological Patterns, Functions and Pathways From Metabolomic Data Using MetaboAnalyst

Jianguo Xia et al. Nat Protoc. .

Abstract

MetaboAnalyst is an integrated web-based platform for comprehensive analysis of quantitative metabolomic data. It is designed to be used by biologists (with little or no background in statistics) to perform a variety of complex metabolomic data analysis tasks. These include data processing, data normalization, statistical analysis and high-level functional interpretation. This protocol provides a step-wise description on how to format and upload data to MetaboAnalyst, how to process and normalize data, how to identify significant features and patterns through univariate and multivariate statistical methods and, finally, how to use metabolite set enrichment analysis and metabolic pathway analysis to help elucidate possible biological mechanisms. The complete protocol can be executed in approximately 45 min.

Similar articles

See all similar articles

Cited by 272 articles

See all "Cited by" articles

References

    1. Bioinformatics. 2008 Mar 1;24(5):732-7 - PubMed
    1. Anal Chem. 2009 May 1;81(9):3623-9 - PubMed
    1. Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50 - PubMed
    1. Nucleic Acids Res. 2009 Jul;37(Web Server issue):W115-21 - PubMed
    1. Nucleic Acids Res. 2007 Jan;35(Database issue):D521-6 - PubMed

Publication types

LinkOut - more resources

Feedback