Analysis of serum adenosine deaminase (ADA) and ADA1 and ADA2 isoenzyme activities in HIV positive and HIV-HBV co-infected patients

Clin Biochem. 2011 Aug;44(12):980-3. doi: 10.1016/j.clinbiochem.2011.05.020. Epub 2011 May 25.


Objectives: To determine adenosine deaminase (ADA) activity as a possible diagnostic marker in HIV and HIV-HBV co-infected patients.

Design and methods: Blood samples were collected from 72 healthy, 33 HIV positive and 30 HIV-HBV co-infected subjects. Blood CD4+ cell count was recorded and serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total ADA, and ADA1 and ADA2 isoenzyme activities were determined.

Results: Serum ALT, AST, total ADA and ADA2 isoenzyme activities were significantly higher in HIV positive and HIV-HBV co-infected groups compare to the control (p<0.05), whereas serum ALP showed no differences between groups. CD4+ cell counts markedly decreased in all patients and showed a significant inverse correlation with ADA activities (R(2)=0.589, p<0.001).

Conclusions: Serum ADA was significantly increased in HIV and HIV-HBV co-infections. Therefore, because of its low cost and simplicity to perform, ADA activity might be considered as a useful diagnostic tool among the other markers in these diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Deaminase / blood*
  • Adult
  • Alanine Transaminase / blood
  • Aspartate Aminotransferases / blood
  • CD4 Lymphocyte Count
  • Case-Control Studies
  • Female
  • HIV Infections / blood
  • HIV Infections / diagnosis
  • HIV Infections / enzymology*
  • HIV Seropositivity / blood
  • HIV Seropositivity / enzymology*
  • Hepatitis B / blood
  • Hepatitis B / enzymology*
  • Humans
  • Isoenzymes / blood
  • Male
  • Middle Aged


  • Isoenzymes
  • Aspartate Aminotransferases
  • Alanine Transaminase
  • ADA protein, human
  • Adenosine Deaminase