Repair of single nucleotide DNA mismatches transfected into mammalian cells can occur by short-patch excision

Mutat Res. 1990 Jul;236(1):59-66. doi: 10.1016/0921-8777(90)90033-2.

Abstract

Synthetic DNA linkers containing a single mismatched nucleotide (C:A) are repaired without bias at high efficiency when introduced into mammalian cells on a SV40 shuttle vector. From the pattern of repair in vectors containing multiple linkers, it appears that DNA synthesis following mismatch excision can replace a length of DNA as short as 40 nucleotides. Furthermore, results from the introduction of linker molecules containing combinations of single-strand nicks suggest that transient unsealed nicks do not drive the direction of mismatch repair in mammalian cells, as has previously been proposed.

MeSH terms

  • Animals
  • Base Composition
  • Base Sequence
  • Cell Line
  • DNA Repair*
  • DNA Replication*
  • Genetic Vectors
  • Molecular Sequence Data
  • Oligonucleotide Probes / chemical synthesis
  • Plasmids
  • Restriction Mapping
  • Simian virus 40 / genetics
  • Transfection*

Substances

  • Oligonucleotide Probes