BAD-LAMP is a novel biomarker of nonactivated human plasmacytoid dendritic cells

Blood. 2011 Jul 21;118(3):609-17. doi: 10.1182/blood-2010-11-319699. Epub 2011 Jun 3.

Abstract

The brain and dendritic cell (BAD)-associated lysosome-associated membrane protein (LAMP)-like molecule (BAD-LAMP, c20orf103, UNC-46) is a newly identified member of the family of LAMPs. BAD-LAMP expression in the mouse is confined to neurons. We demonstrate here that in humans, BAD-LAMP can specifically be found in the type I IFN-producing plasmacytoid dendritic cells (pDCs). Human BAD-LAMP is localized in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of freshly isolated CD123(+) pDCs and is rapidly lost upon activation by unmethylated cytosine-phosphate-guanine (CpG) oligonucleotides. The restricted pattern of BAD-LAMP expression allows for the rapid identification of normal and leukemic human pDCs in tissues and blood.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / metabolism*
  • Brain / metabolism
  • Dendritic Cells / cytology
  • Dendritic Cells / metabolism*
  • Endoplasmic Reticulum / metabolism
  • Endosomes / metabolism
  • Golgi Apparatus / metabolism
  • HeLa Cells
  • Humans
  • Interleukin-3 / metabolism
  • Interleukin-3 Receptor alpha Subunit / metabolism
  • Leukemia / metabolism
  • Leukemia / pathology*
  • Lysosome-Associated Membrane Glycoproteins / genetics
  • Lysosome-Associated Membrane Glycoproteins / metabolism*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Membrane Transport Proteins / genetics
  • Membrane Transport Proteins / metabolism
  • Oligonucleotide Array Sequence Analysis
  • Oligonucleotides / genetics
  • Oligonucleotides / metabolism
  • Transfection

Substances

  • Biomarkers
  • IL3RA protein, human
  • Interleukin-3
  • Interleukin-3 Receptor alpha Subunit
  • LAMP5 protein, human
  • Lysosome-Associated Membrane Glycoproteins
  • Membrane Proteins
  • Membrane Transport Proteins
  • Oligonucleotides
  • UNC93B1 protein, human