Abstract
Cultured rat sympathetic neurons die within 48 h after being deprived of nerve growth factor. Addition of interferons (IFN-alpha/beta or IFN-gamma) prevented the cell death in a dose-dependent manner. Upon longer periods of nerve growth factor deprivation, IFNs failed to maintain survival. Thus, IFNs retarded neuronal death, but did not prevent it. Ligand binding, autoradiography, and cross-linking experiments demonstrated the presence of specific IFN-gamma receptors on sympathetic neurons similar to those seen on other cell types. The possible relationships of the death-suppressing actions of IFNs are compared to the mechanisms of the antiviral or antiproliferative actions of IFNs.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenylate Kinase / metabolism
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Animals
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Antibodies, Monoclonal / pharmacology
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Cell Survival
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Cells, Cultured
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Ganglia, Sympathetic / cytology*
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Ganglia, Sympathetic / embryology
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Interferon Type I / pharmacology*
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Interferon-gamma / pharmacology*
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Nerve Growth Factors / immunology
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Nerve Growth Factors / physiology*
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Nerve Tissue Proteins / biosynthesis
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Neurons / cytology*
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Neurons / metabolism
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Rats
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Receptors, Cell Surface / metabolism
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Receptors, Immunologic / metabolism
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Receptors, Interferon
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Receptors, Nerve Growth Factor
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Recombinant Proteins
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Time Factors
Substances
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Antibodies, Monoclonal
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Interferon Type I
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Nerve Growth Factors
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Nerve Tissue Proteins
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Receptors, Cell Surface
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Receptors, Immunologic
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Receptors, Interferon
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Receptors, Nerve Growth Factor
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Recombinant Proteins
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Interferon-gamma
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Adenylate Kinase