Herpes Simplex Virus IgG Fc Receptors Induced Using Recombinant Adenovirus Vectors Expressing Glycoproteins E and I

Virology. 1990 Aug;177(2):437-44. doi: 10.1016/0042-6822(90)90507-n.

Abstract

Evidence has been presented that herpes simplex virus (HSV) immunoglobulin (IgG) Fc receptors are composed of a complex of two glycoproteins, gE and gI. In previous studies, cells infected with HSV-1 mutants lacking either gE or gI bound lower levels of soluble IgG than cells infected with wild-type viruses suggesting that both gE and gI were required for IgG binding. We have reevaluated the Fc receptor activity of these mutants using a more sensitive assay involving IgG-coated erythrocytes and have found that cells infected with a gE- mutant HSV-1 did not bind IgG-coated erythrocytes whereas cells infected with a gI- mutant retained some Fc binding activity. To further study HSV-induced Fc receptors recombinant adenovirus vectors expressing gE or gI were constructed. Cells expressing gE alone bound both soluble IgG and IgG-coated red cells, although the binding was consistently lower than that observed with HSV-infected cells or cells expressing both gE and gI. Cells expressing only gI were unable to bind either soluble IgG or IgG-coated erythrocytes. These results support the conclusion that both gE and gI are required for full Fc receptor activity, although gE alone can bind IgG to a lesser extent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Antibodies, Monoclonal
  • Antigens, Differentiation / genetics*
  • Antigens, Differentiation / metabolism
  • Cell Line
  • Cell Membrane / immunology
  • Gene Expression
  • Genetic Vectors
  • Immunoglobulin G / metabolism
  • Mutation
  • Plasmids
  • Receptors, Fc / genetics*
  • Receptors, Fc / metabolism
  • Receptors, IgG
  • Restriction Mapping
  • Simplexvirus / genetics*
  • Simplexvirus / immunology
  • Vero Cells
  • Viral Envelope Proteins / genetics*
  • Viral Envelope Proteins / immunology

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation
  • Immunoglobulin G
  • Receptors, Fc
  • Receptors, IgG
  • Viral Envelope Proteins
  • glycoprotein E, herpes simplex virus type 1
  • glycoprotein I, herpes simplex virus type 1