Activity-dependent neuroprotective protein modulates its own gene expression

J Mol Neurosci. 2012 Jan;46(1):33-9. doi: 10.1007/s12031-011-9562-y. Epub 2011 Jun 7.

Abstract

We investigated whether activity-dependent neuroprotective protein (ADNP) could autoregulate its own expression. Both the endogenous ADNP gene and reporter gene constructs were analysed in response to overexpression of ADNP, supplied either as wild-type ADNP or a mutant form lacking the NAP motif, a motif which has neuroprotective properties. Overexpression of these two forms of ADNP resulted in both decreased endogenous ADNP expression and repressed ADNP promoter-directed reporter gene activity. Chromatin immunoprecipitation demonstrated the ability of ADNP to bind to its own promoter which is consistent with its action as a repressor of both promoter-supported and endogenous ADNP expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Gene Expression Regulation / physiology*
  • Genes, Reporter / genetics
  • HeLa Cells
  • Homeodomain Proteins / genetics*
  • Humans
  • Nerve Tissue Proteins / genetics*
  • Neurons / physiology*
  • Promoter Regions, Genetic / genetics

Substances

  • ADNP protein, human
  • Homeodomain Proteins
  • Nerve Tissue Proteins