Gene expression profile of the regeneration epithelium during axolotl limb regeneration

Dev Dyn. 2011 Jul;240(7):1826-40. doi: 10.1002/dvdy.22669. Epub 2011 Jun 3.


Urodele amphibians are unique among adult vertebrates in their ability to regenerate missing limbs. The process of limb regeneration requires several key tissues including a regeneration-competent wound epidermis called the regeneration epithelium (RE). We used microarray analysis to profile gene expression of the RE in the axolotl, a Mexican salamander. A list of 125 genes and expressed sequence tags (ESTs) showed a ≥1.5-fold expression in the RE than in a wound epidermis covering a lateral cuff wound. A subset of the RE ESTs and genes were further characterized for expression level changes over the time-course of regeneration. This study provides the first large scale identification of specific gene expression in the RE.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Ambystoma mexicanum / genetics
  • Ambystoma mexicanum / physiology*
  • Amphibian Proteins / genetics
  • Animals
  • Epithelium / metabolism
  • Epithelium / physiology*
  • Gene Expression Profiling / methods*
  • In Situ Hybridization
  • Oligonucleotide Array Sequence Analysis
  • Regeneration / genetics
  • Regeneration / physiology*


  • Amphibian Proteins