Multicentric evaluation of new commercial enzyme immunoassays for the detection of immunoglobulin M and total antibodies against hepatitis A virus

M C Arcangeletti; E Dussaix; F Ferraglia; A M Roque-Afonso; A Graube; C Chezzi

doi:10.1128/CVI.00403-10

Multicentric evaluation of new commercial enzyme immunoassays for the detection of immunoglobulin M and total antibodies against hepatitis A virus

Clin Vaccine Immunol. 2011 Aug;18(8):1391-4. doi: 10.1128/CVI.00403-10. Epub 2011 Jun 8.

Authors

M C Arcangeletti¹, E Dussaix, F Ferraglia, A M Roque-Afonso, A Graube, C Chezzi

Affiliation

¹ Microbiology Section, Department of Pathology and Laboratory Medicine, University of Parma, Viale Antonio Gramsci 14, 43126 Parma, Italy. mariacristina.arcangeletti@unipr.it

Abstract

A multicentric clinical study was conducted on representative sera from 1,738 European and U.S. subjects for the evaluation of new anti-hepatitis A virus enzyme immunoassays from Bio-Rad Laboratories. Comparison with reference DiaSorin S.p.A. tests confirmed the good performance of Bio-Rad assays (99.85% and 99.47% overall agreement in detecting total antibodies and IgM, respectively).

Publication types

Comparative Study
Evaluation Study
Multicenter Study
Research Support, Non-U.S. Gov't

MeSH terms

Clinical Laboratory Techniques / methods*
Hepatitis A / diagnosis*
Hepatitis A Antibodies / blood*
Hepatitis A virus / immunology*
Humans
Immunoenzyme Techniques / methods
Immunoglobulin M / blood*

Substances

Hepatitis A Antibodies
Immunoglobulin M