The effect of hyperbaric oxygen treatment on aspiration pneumonia

J Mol Histol. 2011 Aug;42(4):301-10. doi: 10.1007/s10735-011-9334-6. Epub 2011 Jun 8.


We have studied whether hyperbaric oxygen (HBO) prevents different pulmonary aspiration materials-induced lung injury in rats. The experiments were designed in 60 Sprague-Dawley rats, ranging in weight from 250 to 300 g, randomly allotted into one of six groups (n = 10): saline control, Biosorb Energy Plus (BIO), hydrochloric acid (HCl), saline + HBO treated, BIO + HBO treated, and HCl + HBO treated. Saline, BIO, HCl were injected into the lungs in a volume of 2 ml/kg. A total of seven HBO sessions were performed at 2,4 atm 100% oxygen for 90 min at 6-h intervals. Seven days later, rats were sacrificed, and both lungs in all groups were examined biochemically and histopathologically. Our findings show that HBO inhibits the inflammatory response reducing significantly (P < 0.05) peribronchial inflammatory cell infiltration, alveolar septal infiltration, alveolar edema, alveolar exudate, alveolar histiocytes, interstitial fibrosis, granuloma, and necrosis formation in different pulmonary aspiration models. Pulmonar aspiration significantly increased the tissue HP content, malondialdehyde (MDA) levels and decreased (P < 0.05) the antioxidant enzyme (SOD, GSH-Px) activities. HBO treatment significantly (P < 0.05) decreased the elevated tissue HP content, and MDA levels and prevented inhibition of SOD, and GSH-Px (P < 0.05) enzymes in the tissues. Furthermore, there is a significant reduction in the activity of inducible nitric oxide synthase, TUNEL and arise in the expression of surfactant protein D in lung tissue of different pulmonary aspiration models with HBO therapy. It was concluded that HBO treatment might be beneficial in lung injury, therefore, shows potential for clinical use.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Glutathione Peroxidase / metabolism
  • Hydroxyproline / metabolism
  • Hyperbaric Oxygenation*
  • In Situ Nick-End Labeling
  • Malondialdehyde / metabolism
  • Nitric Oxide Synthase Type II / metabolism
  • Pneumonia, Aspiration / metabolism
  • Pneumonia, Aspiration / pathology
  • Pneumonia, Aspiration / therapy*
  • Rats
  • Rats, Sprague-Dawley
  • Superoxide Dismutase / metabolism


  • Malondialdehyde
  • Glutathione Peroxidase
  • Nitric Oxide Synthase Type II
  • Superoxide Dismutase
  • Hydroxyproline