Efficient transformation of Agrobacterium tumefaciens by electroporation

M Mersereau; G J Pazour; A Das

doi:10.1016/0378-1119(90)90452-w

Efficient transformation of Agrobacterium tumefaciens by electroporation

Gene. 1990 May 31;90(1):149-51. doi: 10.1016/0378-1119(90)90452-w.

Authors

M Mersereau¹, G J Pazour, A Das

Affiliation

¹ Department of Biochemistry, University of Minnesota, St. Paul 55108.

PMID: 2165971
DOI: 10.1016/0378-1119(90)90452-w

Abstract

High-voltage electroporation was used to transform Agrobacterium tumefaciens strains A136 and A348, reaching the efficiency of 1-3 x 10(8) transformants/micrograms DNA. Transformation frequency was dependent on the electrical field strength and the pulse length. No significant reduction in transformation efficiency was observed when the transforming DNA contained sites sensitive to endonuclease AtuCI of A. tumefaciens.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

DNA Restriction Enzymes / metabolism
Deoxyribonucleases, Type II Site-Specific / metabolism
Electricity
Genetic Vectors
Plasmids
Rhizobium / genetics*
Transformation, Genetic*

Substances

DNA Restriction Enzymes
endodeoxyribonuclease BclI
Deoxyribonucleases, Type II Site-Specific

Grants and funding

GM 37555/GM/NIGMS NIH HHS/United States