Efficient transformation of Agrobacterium tumefaciens by electroporation

Gene. 1990 May 31;90(1):149-51. doi: 10.1016/0378-1119(90)90452-w.


High-voltage electroporation was used to transform Agrobacterium tumefaciens strains A136 and A348, reaching the efficiency of 1-3 x 10(8) transformants/micrograms DNA. Transformation frequency was dependent on the electrical field strength and the pulse length. No significant reduction in transformation efficiency was observed when the transforming DNA contained sites sensitive to endonuclease AtuCI of A. tumefaciens.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA Restriction Enzymes / metabolism
  • Deoxyribonucleases, Type II Site-Specific / metabolism
  • Electricity
  • Genetic Vectors
  • Plasmids
  • Rhizobium / genetics*
  • Transformation, Genetic*


  • DNA Restriction Enzymes
  • endodeoxyribonuclease BclI
  • Deoxyribonucleases, Type II Site-Specific