Osteogenic and angiogenic effects of mesenchymal stromal cells with co-transfected human Ang-1 gene and BMP2 gene

Biotechnol Lett. 2011 Oct;33(10):1933-8. doi: 10.1007/s10529-011-0654-0. Epub 2011 Jun 10.

Abstract

To increase the osteogenic and angiogenic effects of marrow-derived mesenchymal stromal cells (MSCs), we co-transfected (by means of lentivirus) the human angiopoietin-1 gene (hAng-1) and human bone morphogenetic protein 2 gene (hBMP2) into MSCs. Real-time PCR and ELISA showed that both genes were successfully co-expressed in the MSCs with expression sustained until the eighth week. The alkaline phosphatase activity of the MSCs was more significantly augmented by the co-transfection with both genes than by any single gene transfection. These results demonstrate that the combined gene therapy with hAng-1 and hBMP2 using lentivirally co-transfected MSCs is feasible.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Analysis of Variance
  • Angiopoietin-1 / biosynthesis
  • Angiopoietin-1 / genetics*
  • Animals
  • Biomarkers / metabolism
  • Bone Morphogenetic Protein 2 / biosynthesis
  • Bone Morphogenetic Protein 2 / genetics*
  • Cell Line, Tumor
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Mesenchymal Stem Cells / metabolism*
  • Neovascularization, Physiologic
  • Osteogenesis
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Rats
  • Rats, Wistar
  • Real-Time Polymerase Chain Reaction
  • Transfection

Substances

  • ANGPT1 protein, human
  • Angiopoietin-1
  • BMP2 protein, human
  • Biomarkers
  • Bone Morphogenetic Protein 2
  • RNA, Messenger
  • Alkaline Phosphatase