Cell biology of homologous recombination in yeast

Methods Mol Biol. 2011;745:523-36. doi: 10.1007/978-1-61779-129-1_30.

Abstract

Homologous recombination is an important pathway for error-free repair of DNA lesions, such as single- and double-strand breaks, and for rescue of collapsed replication forks. Here, we describe protocols for live cell imaging of single-lesion recombination events in the yeast Saccharomyces cerevisiae using fluorescence microscopy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Breaks, Double-Stranded
  • DNA Damage / genetics
  • Microscopy, Fluorescence
  • Recombination, Genetic / genetics*
  • Saccharomyces cerevisiae / genetics