Glycine receptors influence radial migration in the embryonic mouse neocortex

Neuroreport. 2011 Jul 13;22(10):509-13. doi: 10.1097/WNR.0b013e328348aafe.

Abstract

To investigate whether glycine receptors influence radial migration in the neocortex, we analyzed the effect of glycine and the glycinergic antagonist strychnine, on the distribution of 5-bromo-2'deoxyuridine-labeled neurons in organotypic slice cultures from embryonic mice cortices. Application of glycine impeded radial migration only in the presence of the glycine-transport blockers, ALX-5407 and ALX-1393. This effect was blocked by the specific glycine receptor antagonist strychnine, whereas application of strychnine in the absence of glycine was without effect. We conclude from these observations that an activation of glycine receptors can impede radial migration, but that the glycinergic system is not directly implicated in the regulation of radial migration in organotypic slice cultures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bromodeoxyuridine / metabolism
  • Cell Count
  • Cell Movement / drug effects
  • Cell Movement / physiology*
  • Drug Interactions
  • Embryo, Mammalian
  • Glial Fibrillary Acidic Protein / metabolism
  • Glycine / pharmacology
  • Glycine Agents / pharmacology
  • Mice
  • Neocortex / cytology*
  • Neurons / drug effects
  • Neurons / physiology*
  • Organ Culture Techniques
  • Phosphopyruvate Hydratase / metabolism
  • Receptors, Glycine / metabolism*
  • Sarcosine / analogs & derivatives
  • Sarcosine / pharmacology
  • Strychnine / pharmacology

Substances

  • (R)-(N-(3-(4'-fluorophenyl)-3-(4'-phenylphenoxy)propyl))sarcosine
  • Glial Fibrillary Acidic Protein
  • Glycine Agents
  • Receptors, Glycine
  • Phosphopyruvate Hydratase
  • Bromodeoxyuridine
  • Strychnine
  • Glycine
  • Sarcosine