Dectin-1 mediates in vitro phagocytosis of Candida albicans yeast cells by retinal microglia

FEMS Immunol Med Microbiol. 2011 Oct;63(1):148-50. doi: 10.1111/j.1574-695X.2011.00829.x. Epub 2011 Jul 8.

Abstract

We have investigated the expression of TLR2 and Dectin-1 in retinal microglia and their involvement in Candida albicans phagocytosis using a cytometric approach. The expression of both receptors has been demonstrated in CD11b(+) retinal cells. Phagocytosis of pHrodo-labelled C. albicans yeasts by microglial CD11b(+) cells of C57BL/6 mice was inhibited both by the Dectin-1 antagonist laminarin and anti-Dectin-1 antibodies, whereas phagocytosis of yeasts by retinal microglia of TLR2 KO mice was unaffected. These data indicate that phagocytosis of C. albicans yeasts by retinal microglia is mediated by Dectin-1, whereas TLR2 does not play a significant role in this process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD11 Antigens / analysis
  • Candida albicans / immunology*
  • Gene Expression Profiling
  • Lectins, C-Type
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / immunology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microglia / chemistry
  • Microglia / immunology*
  • Microglia / microbiology
  • Nerve Tissue Proteins / biosynthesis
  • Nerve Tissue Proteins / immunology*
  • Phagocytosis*
  • Retina / immunology*
  • Retina / microbiology
  • Toll-Like Receptor 2 / biosynthesis
  • Toll-Like Receptor 2 / immunology

Substances

  • CD11 Antigens
  • Lectins, C-Type
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Tlr2 protein, mouse
  • Toll-Like Receptor 2
  • dectin 1